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2025
- Book : 10(4)
- Pub. Date : 2025
- Page : pp.101749-101749
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2025
- Book : ()
- Pub. Date : 2025
- Page : pp.101773-101773
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2025
Objective: Calcific aortic valve disease (CAVD) is a progressive cardiovascular condition driven by the osteogenic differentiation of valve interstitial cells (VICs), with no effective drug therapies currently available. Hence, our objective is to investigate the impact of thrombospondin‐1 (TSP‐1) silencing on CAVD progression.Methods: In vitro experiments were employed using human primary VICs with TSP‐1 knockdown, cultured in osteogenic induction medium, and followed by analyses including western blot, alkaline phosphatase staining, alizarin red staining, immunofluorescence, and flow cytometry. In vivo experiments used two murine models of CAVD to determine the role of TSP‐1 silencing on aortic valve calcification.Results: We observed that silencing of TSP‐1 reduced the osteogenic differentiation of VICs. Subsequent experiments demonstrated that TSP‐1 knockdown suppressed nuclear factor‐κB (NF‐κB)–mediated inflammation during osteoblastic differentiation of VICs. Consistent findings were also observed in two murine models of CAVD.Conclusions: The present study has shown that TSP‐1 silencing could mitigate the development of CAVD by inhibiting NF‐κB‐mediated inflammation. We propose that targeting TSP‐1‐mediated NF‐κB pathway could provide a potential therapeutic method for treating CAVD.- Book : 2025(1)
- Pub. Date : 2025
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2025
Inflammatory bowel disease (IBD) is a chronic disease influenced by a complex interplay of factors, including genetics, environmental, and gut microbiota. This study aimed to explore the therapeutic potential of the natural polyphenolic compound hydroxytyrosol (HT) in modulating dextran sodium sulfate (DSS)-induced colitis in mice. The findings demonstrate that oral administration of HT significantly alleviated colitis symptoms, as evidenced by a reduction in the disease activity index and improvements in colonic pathology. HT was found to inhibit the release of pro-inflammatory cytokines, enhance antioxidant status, and mitigate oxidative stress. Furthermore, HT contributed to the restoration of the gut barrier by reinstating tight junction proteins, reducing the inflammatory marker lipopolysaccharide (LPS), and suppressing inflammation-related genes. This compound also modulated the NLRP3-Cas-1-GSDMD-IL-1β inflammatory pathway and inhibited the NF-κB (nuclear factor kappa B) pathway, thereby alleviating colitis. Gut microbial analysis revealed that HT enriched the abundance of Bacteroidota and altered the balance between Bacteroidota and Firmicutes in mice. Correlation analysis between bacterial microbiota and inflammatory factors suggested that HT may alleviate colitis by modulating the relative abundance of Alistipes, Bacteroides, and unclassified_f__Muribaculaceae. These findings underscore the potential of HT as a therapeutic agent in the treatment of colitis.- Book : 14(7)
- Pub. Date : 2025
- Page : pp.1270-1270
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2025
Abstract
We investigated the emission of electromagnetic waves from the stainless-steel hollow needle to mesh corona and quasi-periodical spark discharge without and with airflow through the negative needle electrode in the frequency range below 500 MHz. We showed that there is no electromagnetic waves emission from the pulseless corona discharge. After the discharge transition to the quasi-periodical spark, high-amplitude current pulses correlate with the bursts of electromagnetic radiation. We demonstrated that the spectral distribution of emitted radiation is independent of the discharge voltage, current, and airflow through the needle electrode. We found that the highest peak of the power level of the electric component of emitted waves occurs at the frequency of 15.2 MHz, and the power level of emitted radiation as a function of frequency gradually decreases.
Graphical abstract
- Book : 79(4)
- Pub. Date : 2025
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2025
Abstract
Although anti-nuclear antibodies (ANA) are considered the main entry criteria for a diagnosis of undifferentiated connective tissue disease (UCTD), many patients show different rate of ANA positivity and questionable diagnoses. Aim of the study was to revise the UCTD diagnoses and analyse the main predictors of evolution in a monocentric cohort. We retrospectively revised the diagnoses of 331 ANA positive patients, with at least one year of follow-up, classified as UCTD from 2009 and 2017. The diagnosis of UCTD was confirmed in 180 cases (54.4%). The evolution occurred in 18% of cases, after a follow-up of 6.9 (SD: 4.4) years. Raynaud’s phenomenon (RP) (OR: 2.39), puffy hands (OR: 6.3), anti-ENA (OR: 2.34), anti-Topoisomerase I antibodies (OR: 4.93), rheumatoid factor (RF) (OR: 2.86) were associated with evolution. Evolution in Systemic Lupus Erythematosus (SLE) occurred in 5 patients (2.78%) and associated with the addition of new autoantibodies, compared with other evolutions (p: 0.034; OR: 12; 95CI: 1.4–103.4). Evolution in Systemic Sclerosis and pSS was found in 14 (7.8%) and 8 cases (4.4%), respectively. Puffy hands and RF positivity as the predictors of SSc and pSS evolution, respectively. A confirmed diagnosis of UCTD, according with the available criteria, was assessed in about a half patients. The occurrence of puffy hands since the onset defines a patient with a potential evolution into SSc, while the addition of new specific autoantibodies represents a typical “fingerprint” of patients developing SLE.
Trial registration: Studio ANACTD np 1318.- Book : 25(1)
- Pub. Date : 2025
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2025
ABSTRACTObjectiveSurvivin is used to determine the prognosis and clinical features of premalignant and malignant lesions. The aim of this study was to determine the correlation between the expression of survivin isoforms and clinical outcomes in oral lichen planus and oral squamous cell carcinoma.Materials and MethodsThis cross‐sectional study examined 119 cases, including oral squamous cell carcinoma (SCC), oral lichen planus (OLP), and healthy margins of lesions. For all lesions, survivin expression was assessed quantitatively and qualitatively using real‐time polymerase chain reaction. The data were analyzed using SPSS 20.ResultsThe expression of survivin‐∆EX3 and survivin‐2B were quantitatively and qualitatively higher in SCC and OLP cases than in healthy mucosa (p < 0.05). The mean expression of survivin‐∆EX3 in erosive OLP (4.95 ± 4.41) was higher than that in nonerosive OLP (2.13 ± 3.32, p < 0.05). Moreover, the mean expression of both genes was significantly higher in different grades of SCC compared to healthy mucosa (p < 0.05). There was also a significant correlation between gene expressions (p < 0.001).ConclusionThe increased expression of survivin‐∆EX3 and survivin‐2B in OSCC correlates with tumor progression and advanced clinical stages, suggesting a potential prognostic role.- Book : 11(1)
- Pub. Date : 2025
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2025
- Book : 14(2)
- Pub. Date : 2025
- Page : pp.387-391
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2025
Abstract
In the process of spherical near-field measurements for antennas, incomplete near-field data acquisition due to obstructions in certain regions can significantly affect the accuracy of the antenna radiation pattern test results. To address the far-field pattern errors caused by missing data, an inverse distance weighting interpolation method is proposed to restore the missing near-field data before performing the near-to-far-field transformation, thereby reducing the impact of missing data on the far-field results. The far-field radiation pattern after data restoration shows a significant improvement compared to before restoration, with average error reductions of 2.6707 dB in the E-plane and 1.5108 dB in the H-plane. Additionally, the obtained far-field radiation pattern closely matches the simulated pattern, indicating that this method is effective in restoring missing near-field data.- Book : 2991(1)
- Pub. Date : 2025
- Page : pp.012027-012027
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2025
By taking silt clay as the research object, two-phase electrode resistivity tests under different water content and dry density conditions were carried out to clarify the resistivity variation law and influence mechanism of silt clay. The results show that the resistivity of the soil decreases sharply in the low moisture content section then tends to stabilize with the change of moisture content, and decreases continuously with the increase of dry density. There are three phases of a medium, namely soil, water and air, in unsaturated soil, so there are mainly three conductive paths: between soil particles, between pore fluids, and between soil-water coupling. Under different moisture content and dry density conditions, there are obvious differences in the effective contact area, and the types and numbers of conductive paths, which in turn affect the resistivity of the soil. The water status and pore structure of the silt clay samples were analyzed by hydrogen nuclear magnetic resonance (1H-NMR) results to clarify the conductive mechanism of unsaturated silt clay. Finally, a volumetric moisture content and resistivity model is established to unify the effects of moisture content and dry density on resistivity, providing a theoretical reference for the construction and operation safety of silt clay engineering.- Book : 20(4)
- Pub. Date : 2025
- Page : pp.e0319072-e0319072
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