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2025
Di-isopropyl methyl phosphonate (DIMP) has no major commercial uses but is a by-product or a precursor in the synthesis of the nerve agent sarin (GB). Also, DIMP is utilized as a simulant compound for the chemical warfare agents sarin and soman in order to test and calibrate sensitive IMS instrumentation that warns against the deadly chemical weapons. DIMP was measured from 2 ppbv (15 μg m−3) to 500 ppbv in the air using a pocket-held ToF ion mobility spectrometer, model LCD-3.2E, with a non-radioactive ionization source and ammonia doping in positive ion mode. Excellent sensitivity (LoD of 0.24 ppbv and LoQ of 0.80 ppbv) was noticed; the linear response was up to 10 ppbv, while saturation occurred at >500 ppbv. DIMP identification by IMS relies on the formation of two distinct peaks: the monomer M·NH4+, with a reduced ion mobility K0 = 1.41 cm2 V−1 s−1, and the dimer M2·NH4+, with K0 = 1.04 cm2 V−1 s−1 (where M is the DIMP molecule); positive reactant ions (Pos RIP) have K0 = 2.31 cm2 V−1 s−1. Quantification of DIMP at trace levels was also achieved by GC-MS over the concentration range of 1.5 to 150 μg mL−1; using a capillary column (30 m × 0.25 mm × 0.25 μm) with a TG-5 SilMS stationary phase and temperature programming from 60 to 110 °C, DIMP retention time (RT) was ca. 8.5 min. The lowest amount of DIMP measured by GC-MS was 1.5 ng, with an LoD of 0.21 μg mL−1 and an LoQ of 0.62 μg mL−1 DIMP. Our results demonstrate that these methods provide robust tools for both on-site and off-site detection and quantification of DIMP at trace levels, a finding which has significant implications for forensic investigations of chemical agent use and for environmental monitoring of contamination by organophosphorus compounds.- Book : 13(2)
- Pub. Date : 2025
- Page : pp.102-102
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2025
Introduction
Pancreatic cancer is one of the most aggressive cancers. It occurs in men more often than women. The primary therapy for these cancers is surgery; chemotherapy, radiation therapy, hormone therapy, or immunotherapy are also used. More and better treatments are being sought for this disease. The use of PARP inhibitors in the treatment of pancreatic cancer has shown good results, so in this article we have done a review of the results of various studies on this topic. In this review, the results of studies on the use of various PARP inhibitors in pancreatic cancer of different hormonal status are presented.
Purpose
This article aims to give you an overview of the trials that have looked at the effects of different PARP inhibitors in the treatment of pancreatic cancer. PARP inhibitors are a relatively new cancer therapy with good results, so it is important to pay attention.
State of Knowledge
In this article, I used the PubMed database and considered papers from the last 10 years, but most of the information in this review comes from papers published after 2020. I have also taken into account the recommendations of the FDA and the European Medicines Agency on the use of PARP inhibitors.
Conclusions
PARP inhibitors have shown significant effects on pancreatic cancer outcomes. The differences in outcomes depending on the type of cancer, the PARP inhibitor used, and the previous therapies used in a given patient tell us how important it is to individualize therapy in oncology. The findings of the studies presented in this review also point to the need for further research that could focus on identifying patients who may best benefit from treatment with PARP inhibitors, as well as studying synergistic effects in combination with other forms of therapy, such as immunotherapy or chemotherapy. The changes in treatment outcomes that these drugs can bring underscore the importance of exploring new therapeutic strategies in oncology.- Book : 77()
- Pub. Date : 2025
- Page : pp.56992-56992
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2025
Background: Sodium butyrate (NaBu), a short-chain fatty acid, modulates global gene expression through histone deacetylase (HDAC) inhibition, suppressing proliferation and inducing apoptosis in various cancers. Rutin (RUT), a polyphenolic flavonoid found in many plants, exhibits notable anticancer properties. Combining chemotherapeutic agents with natural polyphenols represents a promising strategy for cancer therapy. This study aims to evaluate, for the first time, the potential effects of NaBu and RUT combination therapy on metastatic castration-resistant prostate cancer (mCRPC) cells. Methods: PC-3 cells were treated with varying concentrations of NaBu, RUT, and their combinations. Cell viability was assessed using the WST-1 assay. Based on combination index values, selected treatments were further analyzed for apoptosis (Annexin V assay), intracellular reactive oxygen species (ROS) production, mRNA expression levels, and changes in cell and nuclear morphology. Results: The combined treatment of NaBu and RUT significantly reduced cell viability compared to individual treatments. Enhanced apoptotic induction and elevated ROS levels were observed in combination-treated cells, alongside notable changes in cellular and nuclear morphology and mRNA expression levels. Conclusions: NaBu and RUT combination therapy exhibits a synergistic anticancer effect in mCRPC cells by inhibiting cell viability, inducing apoptosis, and increasing ROS production. These findings suggest a promising therapeutic approach that warrants further investigation to elucidate the underlying molecular mechanisms and assess its potential in preclinical and clinical settings.- Book : 13(2)
- Pub. Date : 2025
- Page : pp.296-296
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2025
AbstractGlioblastoma is the most aggressive primary brain tumor characterized by a dismal prognosis and a profound therapy resistance that is most evident for the mesenchymal molecular subtype of glioblastoma. Targeting vascular endothelial growth factor (VEGF)‐A by the monoclonal antibody bevacizumab, despite failing to improve survival in randomized trials, yields relevant benefits in glioblastoma patients such as reduction of radionecrosis, an adverse event associated with radiotherapy. This demands for continued research to identify optimal combinations of anti‐VEGF‐A and standard therapies for glioblastoma treatment. We show here that blocking VEGF‐A in an immune competent orthotopic glioblastoma mouse model resembling the adverse mesenchymal molecular subtype increases the tumoricidal effect of computed tomography (CT)‐based fractionated radiotherapy and also rectifies irradiation‐induced expression of genes with known association to mesenchymal subtype enrichment as revealed by microarray‐based transcriptome analyses of explanted tumors. VEGF‐A blockade also decreases the expression of myeloid‐cell‐related gene patterns in irradiated tumors and lowers inflammatory response in normal brain tissue after tumor irradiation. Hence, these data both provide a hint how blockade of VEGF‐A increases the effect of radiotherapy in mesenchymal glioblastoma and a mechanistic base for clinical observations reporting reduced incidences of radionecrosis in glioblastoma patients treated with radiotherapy upon concurrent administration of bevacizumab.- Book : ()
- Pub. Date : 2025
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2025
The electrolarynx (EL) is a common device for voice reconstruction in laryngectomy patients, but its mechanical sound source generates significant radiation noise, affecting the naturalness and acceptability of the speech. The parametric acoustic array (PAA), which produces directionally propagated difference-frequency sound waves, presents a promising alternative for creating a more natural glottal-like voice source in the trachea while reducing radiation noise. In this study, we developed a tissue-mimicking phantom to simulate human neck tissue and used a single-transducer-based PAA platform to generate modulated ultrasound signals with glottal waveform characteristics. Ultrasonic microphones captured sound signals fromthe trachea and surrounding air, and signal processing was used to isolate the difference-frequency signals. The results demonstrated that difference-frequency signals were successfully detected in the phantom’s trachea, with time-domain waveforms and frequency spectra closely resembling the designed glottal waveform (Pearson correlation coefficient = 0.9438). Additionally, radiation noise produced by the PAA was significantly lower (23 dB, p < 0.0001) compared to the traditional EL. These findings demonstrate the potential of PAA for voice source reconstruction in laryngectomy patients and suggest its capacity to enhance speech rehabilitation outcomes. Further research is required to refine the frequency range and evaluate clinical applicability.- Book : 25(3)
- Pub. Date : 2025
- Page : pp.802-802
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2025
Abstract
Indoleamine 2, 3-dioxygenase 1 (IDO1) has been recognized as an enzyme involved in tryptophan catabolism with immunosuppressive ability. This study determined to investigate the impact of IDO1 on glioblastoma multiforme (GBM) cells. Here, we showed that the expression of IDO1 was markedly increased in patients with glioma and associated with GBM progression. IDO1 overexpression suppressed ferroptotic cell death, reduced ROS and lipid peroxide generation in GBM cells. IDO1 expression increased the SLC7A11 mRNA stability through FTO-dependent m6A methylation. Mechanistically, IDO1 promoted the AhR expression and nuclear translocation, thus facilitating AhR recruitment at the promoter regions of FTO gene and negatively regulating its transcription. These findings demonstrate that IDO1 facilitates GBM progression by inhibiting SLC7A11-dependent ferroptosis through an IDO1-AhR-FTO axis-mediated m6A methylation mechanism.- Book : 11(1)
- Pub. Date : 2025
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2025
Abstract Downward longwave irradiance DLF is one of the main components of the surface radiation balance (SRB), but its direct measurement is currently limited. Clouds modulate its behavior, and clear-sky DLF0 is predominant in composition of the final DLF value. It is shown that in mid-latitude and tropical atmospheres, DLF0 can be represented as the sum of fluxes from three distinct spectral regions: R1 (λ < 7.5 µm), R2 (7.5 to 14 µm), and R3 (λ > 14 µm). R1 and R3 are closely described by blackbody radiation at screen temperature (Tscr), while R2 exhibits a mean emissivity that primarily depends on total precipitable water (w). It is presented a simple yet consistent physically-based model (hereafter denoted by OLD0), suitable for estimation of DLF0 at ground level. Validation of OLD0 with ground-based data of a worldwide set of 21 stations shows fair accuracy with bias MBE lower than 6 W.m-2 and spread (standard deviation STD) lower than 12 W.m-2 for typical values DLF0 ~ 300-400 W.m-2, compatible with surface pyrgeometer measures. The proposed algorithm outperforms existing methods, achieving a mean bias error (in module |MBE|) of approximately 2.8 W.m-2. In contrast, other widely used algorithms typically exhibit |MBEs| ranging from 8.1 to 15.9 W.m-2.- Book : 40()
- Pub. Date : 2025
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2025
Photoreceptor/retinal degeneration is the major cause of blindness. Induced and inherited mouse models of retinal degeneration are valuable tools for investigating disease mechanisms and developing therapeutic interventions. This study investigated the potential of the antioxidant resveratrol to relieve photoreceptor degeneration using mouse models. Clinical studies have shown a potential association between thyroid hormone (TH) signaling and age-related retinal degeneration. Excessive TH signaling induces oxidative stress/damage and photoreceptor death in mice. C57BL/6 (rod-dominant) and Nrl−/− (cone-dominant) mice at postnatal day 30 (P30) received triiodothyronine (T3) via drinking water (20 µg/mL) with or without concomitant treatment with resveratrol via drinking water (120 µg/mL) for 30 days, followed by evaluation of photoreceptor degeneration, oxidative damage, and retinal stress responses. In experiments using Leber congenital amaurosis model mice, mother Rpe65−/− and Rpe65−/−/Nrl−/− mice received resveratrol via drinking water (120 µg/mL) for 20 days and 10–13 days, respectively, beginning on the day when the pups were at P5, and pups were then evaluated for cone degeneration. Treatment with resveratrol significantly diminished the photoreceptor degeneration induced by T3 and preserved photoreceptors in Rpe65-deficient mice, manifested as preserved retinal morphology/outer nuclear layer thickness, increased cone density, reduced photoreceptor oxidative stress/damage and apoptosis, reduced upregulation of genes involved in cell death/inflammatory responses, and reduced macroglial cell activation. These findings demonstrate the role of oxidative stress in photoreceptor degeneration, associated with TH signaling and Rpe65 deficiency, and support the therapeutic potential of resveratrol/antioxidants in the management of retinal degeneration.- Book : 14(2)
- Pub. Date : 2025
- Page : pp.154-154
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2025
The solar-assisted heat pump drying (SAHPD) system uniquely incorporates solar-heating refrigerant through hot water from solar evacuated tubes, offering distinct advantages. This study analyzed three experimental setups: a heat pump dryer (HPD) without solar assistance, SAHPD configuration 1 (C1-SAHPD) with solar-heated refrigerant at the discharge line, and SAHPD configuration 2 (C2-SAHPD) with solar-heated refrigerant between condensers, both for performance and economic viability. The experiments maintained consistent parameters, including 5.5 kg of Pandan leaf (Pandanus amaryllifolius), an airflow rate of 0.135 kg/s, and a refrigerant operating pressure of 9.65 bar. The SAHPDs operated when the hot water temperature in the storage tank reached between 70°C and 90°C, with a daily average radiation intensity ranging from 0.670 to 1.102 kW/m² for heating the water. The study revealed average coefficients of performance (COPavg) of 5.34, 5.43, and 6.53 for HPD, C1-SAHPD, and C2-SAHPD, respectively. The specific moisture extraction rate (SMER) for HPD was 2.64, while C1-SAHPD and C2-SAHPD had SMERs of 1.88 and 2.71 at solar fractions of 0.34 and 0.45, respectively. Notably, C2-SAHPD reduced electricity consumption by 46%. The payback period for drying 11 kg of Pandan leaves per day was 4.56 months for HPD, 4.32 months for C1-SAHPD, and 3.84 months for C2-SAHPD. The study concluded that C2-SAHPD was the most efficient dryer system for Pandan leaves based on its higher efficiency, SMER, and cost recovery. Additionally, the performance optimization presented in this study contributed to developing a novel technique for classifying dryer technologies.- Book : 37(1)
- Pub. Date : 2025
- Page : pp.349-368
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2025
The present review highlights the role of computed tomography (CT), CT colonography (CTC), and magnetic resonance imaging (MRI) in the diagnosis, staging, response evaluation, and follow-up of colorectal cancer. For a CT scan, prior bowel preparation is required. This is done to enhance imaging of the colon with the use of oral or rectal contrast agents. Negative contrast like air or carbon dioxide are helpful in detecting polyps and masses by distending the colon. Virtual colonoscopy offers a lower-radiation alternative for polyp and cancer detection. Intravenous contrast administration with arterial and venous phase CT images is also important in complete staging of a known case of colon cancer and for evaluation of residual/recurrent disease. With respect to MRI, high-resolution T2-weighted images in multiple planes are important, with diffusion-weighted imaging (DWI) sequences being important for restaging. Intravenous contrast is not generally recommended. Contrast-enhanced CT and MRI are used for nodal and distant metastasis staging, with special attention to the pelvic side wall nodes. Positron emission tomography (PET) CT is to be considered for further evaluation if the findings are unclear and recurrence is suspected.- Book : ()
- Pub. Date : 2025
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