• Book : 212()
• Pub. Date : 2025
• Page : pp.111044
• Keyword :

• Book : 212(1)
• Pub. Date : 2025
• Page : pp.143-156
• Keyword :

Context: Senescence-associated secretory phenotype (SASP) marks the progression of cell senescence, where the cell produces a high level of inflammatory mediators and differentiation regulators. Targeting those mechanisms becomes a clear strategy to slow cellular senescence. Ferulic acid (FA) present in pigmented rice is well-known for its broad biological activity, including anti-inflammation and antioxidants. However, the role of FA as an anti-aging agent and its mechanism is limited. Aims: To evaluate the potential target of FA in modulating cellular aging using computational and in vitro analysis. Methods: Binding of FA to the target proteins analyzed by molecular docking analysis using PyRx and AutoDock Vina software. Lipid accumulation of mouse preadipocytes 3T3-L1 cells was analyzed using oil red O staining. Immunofluorescence staining was performed to analyze the level of peroxisome proliferator-activated receptor-gamma (PPAR-γ) and nuclear factor-kappa B (NF-B) proteins. Results: Several transcription factors and inflammatory mediators were used as targets for molecular docking, including CCAAT-enhancer-binding proteins-alpha (C/EBP-α), PPAR-γ, NF-B, IkappaB kinase-beta (IKK-β), interleukin-6 (IL-6), IL-8, and their respective receptors. Although other protein targets have excellent binding energy and suitable FA-residues interactions, the molecular dynamics analysis showed that PPAR-γ was the most suitable target for FA in controlling the SASP process with high structural integrity and outperformed ligand-protein interaction stability. FA reduces the accumulation of lipids in differentiated preadipocyte cells. Importantly, FA treatment showed increases in the level of PPAR-γ while reducing the NF-B proteins in vitro. Conclusions: Consequently, the FA could perform the anti-aging activity in preadipocyte cells by reducing SASP progression through activation of PPAR-γ signaling.

• Book : 13(2)
• Pub. Date : 2025
• Page : pp.402-415
• Keyword :

• Book : 208()
• Pub. Date : 2025
• Page : pp.109477
• Keyword :

• Book : 603()
• Pub. Date : 2025
• Page : pp.155473
• Keyword :

Abstract

The presented work aims to compare and analyze the impact of the abrasive solution heating process using three different techniques on the etching parameters for the solid-state nuclear track detector (SSNTD) (CN-85). Water bath technique (WB) is using water as a conduction medium of heat transfer to heat the etching solution. The thermal oven technique (TO) is using air as a convection heat transfer medium to heat the etching solution. Finally, the micro-wave technique (MW) as electromagnetic radiation no need for a medium to heat the etching solution. That comparison will be achieved at different irradiation times to an Americium-241 source (241Am) of 5.486 MeV energy and 10 μCi activity as an alpha particle's emitter. Two irradiation times 10 s and 20 s are applied. Small pieces of CN-85 detectors (1 × 1) cm2 were used. CN-85 detector parameters of the etching process, with the three applying techniques (WB, TO, and MW), are estimated and compared. The obtained results from the three techniques are compared. The etching process time is developed to nearly 5 min for MW with heating by radiation mechanism compared to 25-35 min for etching techniques (WB and TO) with heating by conduction and convection mechanisms, respectively. The efficiency of MW technique with range of from 70% to 75% are five times greater than that for WB and TO techniques.

• Book : 11(2)
• Pub. Date : 2025
• Page : pp.021002
• Keyword :

• Book : 603()
• Pub. Date : 2025
• Page : pp.155457
• Keyword :

• Book : 101()
• Pub. Date : 2025
• Page : pp.175-190
• Keyword :

• Book : 211()
• Pub. Date : 2025
• Page : pp.110982
• Keyword :

• Book : 211()
• Pub. Date : 2025
• Page : pp.110896
• Keyword :