• Book : 43(1)
• Pub. Date : 2025
• Page : pp.129-149
• Keyword :

• Book : 242(pe)
• Pub. Date : 2025
• Page : pp.116206
• Keyword :

• Book : 18(1)
• Pub. Date : 2025
• Page : pp.101214
• Keyword :

• Book : 10(1)
• Pub. Date : 2025
• Page : pp.101656
• Keyword :

ABSTRACT Nuclear magnetic resonance (NMR) is a spectroscopy technique widely used by chemists and physicists to determine the chemical structure of molecules that was adapted to generate imaging, known as nuclear magnetic resonance imaging (MRI), which is widely used in medical diagnosis. The importance of NMR in chemistry, physics, medicine, materials, and agriculture has been recognized with several Nobel Prizes in Physics, 1952, Chemistry, 1991 and 2002, and Medicine in 2003. Therefore, NMR can be applied to obtain: i) imaging of the human body, animal and materials; ii) high-resolution spectra to obtain structural and dynamical information of chemicals, materials etc.; and iii) quantitative and qualitative information of chemical composition of products such as food and agricultural products, using low-resolution relaxometry. High-resolution NMR and MRI have been applied in agri-food products, mostly as a research tool as they typically rely on expensive and bulk instruments, which restrict their uses in routine applications. The NMR sensors that have been more frequently used in agri-food products are based on low-resolution or low-field or time-domain NMR (TD-NMR) instruments. These low-cost instruments have been used for qualitative and quantitative analysis of agri-food products such as intact seeds and grains, intact fruits, meat, oils, and processed foods. In this paper, an overview of the NMR techniques and its main instrumentation aspects are presented, and some applications of TD-NMR and MRI in the non-invasive analysis of food, seeds, and others agricultural products are discussed.

• Book : 29(3)
• Pub. Date : 2025
• Page : pp.e280966
• Keyword :

Abstract

Immunostimulant phytogenic feed additives are given great concern for improving fish health, growth, immune responses, and resistance to diseases. This research investigated the impact of dietary propolis (PRO), turmeric (TUR), and their combination on the growth, hematology, antioxidant-immune responses, and their regulating genes in Nile tilapia (Oreochromis niloticus) during Edwardsiella tarda challenge. For 8 weeks, a total number of 320 fish (20.70 ± 0.14 g) were allocated into four groups at random, each with eight replicates (10 fish each). The first group (1st) was given the basal diet (control) without any supplements. The 2nd, 3rd, and 4th groups were supplemented with 1% PRO, 1% TUR, and the mixture (1%PRO + 1%TUR), respectively. The experimental groups were challenged intraperitoneally with E. tarda at a dose of 0.1 mL (1 × 10⁵ CFU) at the termination of the feeding trial, and the fish survival was estimated for an additional 7 days. The results demonstrated that fish-fed diets supplemented with PRO and/or TUR showed higher body weight, condition factor, specific growth rate, feed intake, and feed efficiency utilization than the control group (P < 0.05). The hematological, protein profile, and antioxidant-immune (total antioxidant capacity, lysozymes, and IgM) parameters were substantially improved in the challenged fish fed on PRO and/or TUR diets compared to the challenged non-fed fish. The lipid profile and malondialdehyde were substantially decreased in the challenged fish fed on PRO and/or TUR diets compared to the challenged non-fed group. Notably, a down-turning of nuclear factor kappa B (NFκB) and tumor necrosis factor-alpha (TNF-α) expression with up-turning of nuclear factor erythroid 2-related factor 2 (Nrf2) and transforming growth factor-beta (TGF-β) expression was noticed in the challenged fish fed on PRO and/or TUR diets compared to the challenged non-fed fish. Noteworthy, dietary PRO and/or TUR improved the fish survival during E. tarda challenge. The mixture of PRO and TUR can be added to Nile tilapia diets to enhance their growth, immune response, and resistance to E. tarda. These outcomes help in the sustainable development of the Nile tilapia culture industry.

Graphical Abstract

• Book : 33(1)
• Pub. Date : 2025
• Page : pp.46
• Keyword :

The cell nucleus contains distinct biomolecular condensates that form at specific genetic loci, organize chromosomes in 3D space, and regulate RNA processing. Among these, Cajal bodies (CBs) require key “scaffolding” proteins for their assembly, which is not fully understood. Here, we employ proximity biotinylation, mass spectrometry, and functional screening to comprehensively identify and test the functions of CB components. We document 144 protein interactors of coilin, of which 70 were newly detected, and establish 25 players needed for CB assembly and/or maintenance. Surprisingly, the depletion of nine coilin interactors-mostly constituents of the 60S ribosome (RPLs)-increased CB number and caused subdomains defined by coilin and the survival motor neuron protein (SMN) to merge. These phenotypes were traceable to altered nuclear levels of dimethylarginine. Our data implicate RPL24 and other players in the regulation of CBs by modulating posttranslational modifications. Moreover, the prevalence of transcription factors among the identified components highlights roles for gene activity in CB assembly and nuclear positioning.

• Book : 224(2)
• Pub. Date : 2025
• Page : pp.e202305081
• Keyword :

• Book : 1070(p1)
• Pub. Date : 2025
• Page : pp.170013
• Keyword :

• Book : 1070(p2)
• Pub. Date : 2025
• Page : pp.170005
• Keyword :

• Book : 1070(p1)
• Pub. Date : 2025
• Page : pp.170003
• Keyword :